<?xml version="1.0" encoding="UTF-8"?> <rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:ns0="http://www.cropontology.org/rdf/" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:metadata_def="http://data.bioontology.org/metadata/def/"> <owl:Class rdf:about="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277"> <rdf:type rdf:resource="http://www.w3.org/2002/07/owl#NamedIndividual"/> <rdf:type rdf:resource="http://www.w3.org/2004/02/skos/core#Concept"/> <rdfs:subClassOf rdf:resource="http://www.cropontology.org/rdf/Method"/> <skos:prefLabel xml:lang="en">Metabolite content UPLC and HPLC+MS/MS measurement</skos:prefLabel> <metadata_def:mappingSameURI rdf:resource="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277"/> <metadata_def:mappingLoom>metabolitecontentuplcandhplcmsmsmeasurement</metadata_def:mappingLoom> <skos:definition xml:lang="en">UPLC measurement of sample of ca.100-200mg of fresh mass on RP C18 column. Processing of raw data from UPLC 280/330nm: normalisation by sample mass, differentation[1], correlation optimised warping (COW)[2][3], signal detection. Identification with HPLC-MS/MS on RP C18 column. Expressed as apigenin equivalent per gram of fresh mass.</skos:definition> <skos:broaderTransitive rdf:resource="http://www.cropontology.org/rdf/Method"/> <ns0:method_of rdf:resource="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000276"/> <rdfs:label xml:lang="en">Metabolite content UPLC and HPLC+MS/MS measurement</rdfs:label> </owl:Class> <rdf:Description rdf:about="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000278"> <ns0:scale_of> <rdf:Description rdf:about="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277"> <metadata_def:mappingSameURI rdf:resource="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277"/> </rdf:Description> </ns0:scale_of> </rdf:Description> </rdf:RDF>
<?xml version="1.0" encoding="UTF-8"?>
<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:ns0="http://www.cropontology.org/rdf/" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:metadata_def="http://data.bioontology.org/metadata/def/">
  <owl:Class rdf:about="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277">
    <rdf:type rdf:resource="http://www.w3.org/2002/07/owl#NamedIndividual"/>
    <rdf:type rdf:resource="http://www.w3.org/2004/02/skos/core#Concept"/>
    <rdfs:subClassOf rdf:resource="http://www.cropontology.org/rdf/Method"/>
    <skos:prefLabel xml:lang="en">Metabolite content UPLC and HPLC+MS/MS measurement</skos:prefLabel>
    <metadata_def:mappingSameURI rdf:resource="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277"/>
    <metadata_def:mappingLoom>metabolitecontentuplcandhplcmsmsmeasurement</metadata_def:mappingLoom>
    <skos:definition xml:lang="en">UPLC measurement of sample of ca.100-200mg of fresh mass on RP C18 column. Processing of raw data from UPLC 280/330nm: normalisation by sample mass, differentation[1], correlation optimised warping (COW)[2][3], signal detection. Identification with HPLC-MS/MS on RP C18 column. Expressed as apigenin equivalent per gram of fresh mass.</skos:definition>
    <skos:broaderTransitive rdf:resource="http://www.cropontology.org/rdf/Method"/>
    <ns0:method_of rdf:resource="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000276"/>
    <rdfs:label xml:lang="en">Metabolite content UPLC and HPLC+MS/MS measurement</rdfs:label>
  </owl:Class>
  <rdf:Description rdf:about="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000278">
    <ns0:scale_of>
      <rdf:Description rdf:about="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277">
        <metadata_def:mappingSameURI rdf:resource="http://www.cropontology.org/rdf/POLAPGEN_BARLEY:0000277"/>
      </rdf:Description>
    </ns0:scale_of>
  </rdf:Description>
</rdf:RDF>