{"@context":{"ns0":"http://www.cropontology.org/rdf/","rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","owl":"http://www.w3.org/2002/07/owl#","skos":"http://www.w3.org/2004/02/skos/core#","rdfs":"http://www.w3.org/2000/01/rdf-schema#","metadata_def":"http://data.bioontology.org/metadata/def/"},"@graph":[{"@id":"ns0:POLAPGEN_BARLEY:0000223","@type":["owl:Class","skos:Concept","owl:NamedIndividual"],"rdfs:subClassOf":{"@id":"ns0:Method"},"rdfs:label":{"@language":"en","@value":"Metabolite content UPLC and HPLC+MS/MS measurement"},"skos:definition":{"@language":"en","@value":"UPLC measurement of sample of ca.100-200mg of fresh mass on RP C18 column. Processing of raw data from UPLC 280/330nm: normalisation by sample mass, differentation[1], correlation optimised warping (COW)[2][3], signal detection. Identification with HPLC-MS/MS on RP C18 column. Expressed as apigenin equivalent per gram of fresh mass."},"skos:prefLabel":{"@language":"en","@value":"Metabolite content UPLC and HPLC+MS/MS measurement"},"skos:broaderTransitive":{"@id":"ns0:Method"},"metadata_def:mappingLoom":"metabolitecontentuplcandhplcmsmsmeasurement","metadata_def:mappingSameURI":{"@id":"ns0:POLAPGEN_BARLEY:0000223"},"ns0:method_of":{"@id":"ns0:POLAPGEN_BARLEY:0000222"}},{"@id":"ns0:POLAPGEN_BARLEY:0000224","ns0:scale_of":{"@id":"ns0:POLAPGEN_BARLEY:0000223"}}]}