<?xml version="1.0" encoding="UTF-8"?> <rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:obo_purl="http://purl.obolibrary.org/obo/" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:metadata_def="http://data.bioontology.org/metadata/def/" xmlns:metadata="http://data.bioontology.org/metadata/"> <owl:Class rdf:about="http://purl.obolibrary.org/obo/OBI_0600036"> <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/OBI_0000094"/> <rdfs:label>establishing cell culture</rdfs:label> <rdfs:comment>A 'cell culture' as used here referes to a new lineage of cells in culture deriving from a single biological source.. New cultures are established through the initial isolation and culturing of cells from an organismal source, or through changes in an existing cell culture or line that result in a new culture with unique characteristics. This can occur through the passaging/selection of a primary culture into a secondary culture or line, or experimental modifications of an existing cell culture or line such as an immortalization process or other stable genetic modification. This class covers establishment of cultures of either multicellular organism cells or unicellular organisms.</rdfs:comment> <obo_purl:IAO_0000115>a process through which a new type of cell culture or cell line is created, either through the isolation and culture of one or more cells from a fresh source, or the deliberate experimental modification of an existing cell culture (e.g passaging a primary culture to become a secondary culture or line, or the immortalization or stable genetic modification of an existing culture or line).</obo_purl:IAO_0000115> <obo_purl:IAO_0000117>PERSON:Matthew Brush</obo_purl:IAO_0000117> <obo_purl:IAO_0000111>'establishing cell culture'</obo_purl:IAO_0000111> <obo_purl:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000120"/> <obo_purl:IAO_0000119>PERSON:Matthew Brush</obo_purl:IAO_0000119> <metadata_def:prefLabel>establishing cell culture</metadata_def:prefLabel> <metadata_def:mappingLoom>establishingcellculture</metadata_def:mappingLoom> <metadata_def:mappingSameURI rdf:resource="http://purl.obolibrary.org/obo/OBI_0600036"/> <metadata:prefixIRI>OBI:0600036</metadata:prefixIRI> <obo_purl:OBI_0000293 rdf:resource="http://purl.obolibrary.org/obo/CL_0000000"/> <obo_purl:OBI_0000293 rdf:resource="http://purl.obolibrary.org/obo/OBI_0000079"/> <obo_purl:OBI_0000299 rdf:resource="http://purl.obolibrary.org/obo/OBI_0100060"/> </owl:Class> <rdf:Description rdf:about="http://purl.obolibrary.org/obo/OBI_0600036"> <metadata_def:mappingSameURI rdf:resource="http://purl.obolibrary.org/obo/OBI_0600036"/> </rdf:Description> </rdf:RDF>